CHM373H5

Techniques in Biological Chemistry II

The second in a sequence of two laboratory courses intended to complement CHM361H5 and CHM362H5. CHM373H5 carries on from CHM372H5 with a particular emphasis on protein purification, enzyme kinetics and protein characterization (e.g., kinetics, reactions, binding, depending on the protein studied). Techniques covered include classic biochemical techniques used in studying proteins and protein complexes, such as chromatography and fluorescence methods. The theoretical basis for each experiment will be covered in a 1-hour lecture each week.